Herbicide Resistance And Phosphatase Enzyme Activity Of Phosphate Solubilizing Bacteria In Rhizosphere Soil

This book of study reveals that twenty-five isolates were recovered as phosphate solubilizing bacteria (PSB) from rhizosphere soil of paddy plants from four different locations of paddy field in Alwarkurichi village of Tenkasi District. Phosphate solubilizing bacterial population ranged from 30-50 × 102 CFU/g in Pikovskaya’s agar medium, 20-30 × 102 CFU/g in hydroxy apatite agar medium and 10-20 × 102 CFU/g in soil extract agar medium. Among the tested isolates, highest load of phosphate solubilizers were found as the best in solubilizing phosphate 102 dilutions with the drop in neutral pH in Pikovskaya’s agar medium. The results indicated that the rhizosphere soils of the study area inhabit phosphate solubilizing bacteria with great potential to be used as the commercial production of phosphatase enzymes. The phosphate solubilizing bacteria was composed of Bacillus sp., Micrococcus sp. and with a lone of Pseudomonas sp. Both Bacillus sp. and Micrococcus sp. were noted to produce phosphatase at neutral pH and at 450 C with the exception of Bacillus sp. PS 25. Probably this bacterium is secreting alkaline phosphatase that has temperature optima at 350 C.